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KMID : 0613820180280111369
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Journal of Life Science
2018 Volume.28 No. 11 p.1369 ~ p.1378
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Physiological Activity and Physicochemical Properties of Condensed Prunus mume Juice Prepared with Pectinase
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Kim Jeong-Ho
Cho Hyun-Dong
Won Yeong-Seon
Park Wool-Lim
Lee Kwan-Woo
Kim Hyuk-Joo
Seo Kwon-Il
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Abstract
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Prunus mume Siebold & Zucc., a member of the Rosaceae family (called Maesil in Korea), has been widely distributed in East Asia, e.g. Korea, Japan and China, and its fruit has been used as a traditional drug and health food. In this study, we evaluated physicochemical properties and physiological activities of condensed Prunus mume juice treated with pectinase (PJ). The values of total acidity, pH, sugar contents, turbidity moisture content of the PJ were 35.81%, 2.73, 54.36˚Brix, 2.75 and 51.32%, respectively. The PJ had effective DPPH radical scavenging activity, reducing power effect, H2O2 scavenging activity and β-carotene bleaching effect. DPPH radical scavenging activities of PJ was 46.31%; their reducing power (OD700) was 1.80; H2O2 scavenging activity of PJ was 91.62%; and β-carotene bleaching effect of PJ was 73.02%. Also, PJ showed effective levels of α-glucosidase inhibition activity. The cell viability was measured by SRB assay. The PJ significantly decreased the cell viability of mouse melanoma cells (B16) and human melanoma cells (SK-MEL-2 and SK-MEL-28) in a dose-dependent manner, however, there was no effect on human keratinocyte HaCaT. In morphological study, PJ-treated SK-MEL-2 cells showed distorted and shrunken cell masses. Total polyphenol contents and total flavonoid contents of PJ were 588.31 mg% (gallic acid equivalent) and 860.45 mg% (rutin equivalent). The antiproliferative effect of PJ seems to be associated with the antioxidant activity of its flavonoid and polyphenol contents. In conclusion, PJ may be beneficial in development of a functional food material.
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KEYWORD
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Antioxidant, antiproliferaion, physicochemical property, Prunus mume, skin cancer
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